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In summary, ADV-NOX2-AS successfully inhibited hypoxia-induced oxidative stress and apoptosis in cardiomyocyte in vitro. This finding indicates that NOX2 may be a targeting candidate for the therapies in heart ischemia diseases.. In the same way, like the previous experiment, Irx3 indirectly promotes Cx40 expression by combining with an unknown factor in the development of conduction system, Irx4 plays an important role in the process of cardiac compartmentation by combination with Vitamin D, the interaction between Irx4, Irx5 and mBop has yet to be clarified, and in addition, in recent years, the understanding of the three-dimensional structure of Irx gene clusters has been further deepened. In the process of verifying the function of individual Irx genes, it also needs to verify the function of the complexes and their structures.

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